目的 建立腹达康微丸中盐酸小檗碱的HPLC含量测定方法。方法 采用Hypersil C18色谱柱(4.6 mm×250 mm，5 μm)，流动相为乙腈-0.03 mol/L磷酸(三乙胺调pH至3.5)(38 ∶ 62)，流速为1.0 mL·min-1，检测波长为265 nm，柱温为室温。结果 盐酸小檗碱线性范围为5.04～60.44 μg·min-1，平均回收率为100.8 %，RSD为2.0 %。结论 该法操作简单，重现性好，结果准确，可作为腹达康微丸的质量控制方法。

Objective To establish a method for the determination of berberine hydrochloride in Fudakang pellets by HPLC. Methods The chromatographic conditions were as follows:Hypersil C18(4.6 mm×250 mm，5 μm) at room temperature，C2H3N-0.03mol/LH3PO4(adjusting the pH value to be 3.5 with triethylamine) in the volume fraction of 32 ∶ 68 as mobile phase，the flow rate at 1.0 mL·min-1，the detection wavelength being 265 nm. Results The calibration curves showed good linearity in the range of 5.04～60.44 μg·mL-1，the average recoveries was 100.8 %，and RSD was 2.0 ％. Conclusion The established method is simple，with exact result and good reproducibility，and can be used for the quality control of Fudakang pellets.

R284.1

广东省科技计划课题(2011B031700079)。