基于血清药理学和网络药理学探究清感童饮的体外抗炎作用。方法采用血清药理学评价方法， 验证清感童饮含药血清对脂多糖（LPS）诱导的小鼠巨噬细胞（RAW264.7）释放一氧化氮（NO）、细胞坏死因子α （TNF-α）、白细胞介素6（IL-6）的影响。利用液质联用（UHPLC-MS/MS）技术对清感童饮指标性成分进行含量 测定，并根据这些成分进行网络药理学分析，对清感童饮有效成分发挥抗炎作用的潜在靶点及作用通路进行预 测。结果细胞实验证明清感童饮可明显降低NO、TNF-α、IL-6 水平（P＜0.05，P＜0.01，P＜0.001）。清感 童饮中含量较高的成分为连翘酯苷A、牛蒡苷、绿原酸、野黄芩苷、没食子酸、迷迭香酸、芍药内酯苷、连翘 苷。清感童饮中17 个活性成分与炎症的交集靶点共215 个，主要涉及ALB、VEGFA、IL-6、TNF-α 等31 个 核心靶点，调节AGE-RAGE、PI3K-Akt、MAPK 信号通路等多种通路发挥抗炎作用。结论清感童饮具有多 成分-多靶点发挥抗炎作用的特点。

To preliminary explore the in vitro anti-inflammatory effects of Qinggan Tongyin based on serum pharmacology and network pharmacology. Methods The effects of the serum containing Qinggan Tongyin on the release of NO，cell necrosis factor-α （TNF-α），and interleukin-6 （IL-6） in LPS-induced RAW264.7 cells were confirmed using serum pharmacology. UHPLC-MS/MS was used to determine the index components of Qinggan Tongyin. The possible targets and pathways of active components in Qinggan Tongyin for anti-inflammatory properties were predicted by using network pharmacology. Results The results of cellular assay showed that Qinggan Tongyin could dramatically lessen the levels of NO，TNF-α，and IL-6（P＜0.05，P＜0.01，P＜0.001）. The higher contents of Qinggan Tongyin were phillyrin A， arctiin， chlorogenic acid， scutellarin， gallic acid， rosmarinic acid， paeoniflorin and phillyrin. A totsl of 215 intersection targets between 17 active components in Qinggan Tongyin and inflammation were obtained，and the 31 core targets were ALB，VEGFA，IL-6，TNF-α，etc. . The primary targets can exhibit anti-inflammatory actions by regulating several signaling pathways，such as AGE-RAGE， PI3K-Akt，and MAPK signaling pathway. Conclusion Qinggan Tongyin exerts its anti-inflammatory effects with the characteristic of multiple components and multiple targets.

R285.5

天津市中医药重点领域科研项目（2021004）。