探讨苦参碱（Matrine）对冠心病（coronary heart disease，CHD）大鼠辅助T 细胞17（helper T cell 17， Th17）/调节性T 细胞（regulatory T cells，Treg）细胞平衡及Ras 同源基因家族成员A（RhoA）-Rho 相关的卷曲螺 旋激酶（ROCK）信号通路的影响。方法建立冠心病模型，将实验大鼠分为对照组、模型组、苦参碱低剂量 （50 mg·kg-1）组、苦参碱高剂量（200 mg·kg-1）组及苦参碱高剂量（200 mg·kg-1）+LPA 组（10 mg·kg-1）。超声心动 图进行大鼠心功能检测；酶联免疫吸附试验（enzyme linked immunosorbent assay，ELISA）法进行白细胞介素17 （IL-17）、转化生长因子β（TGF-β）水平检测；流式细胞术检测Th17、Treg 数量及Th17/Treg 比值；免疫组化 进行内皮型一氧化氮合酶（eNOS）、内皮素1（ET-1）蛋白表达水平检测；Masson 染色进行大鼠心肌组织的病理 形态变化观察；TTC 染色检测各组大鼠心肌梗死情况；TUNEL 染色进行心肌组织中细胞凋亡情况检测；试剂 盒检测RhoA 活性；Western Blot 法进行半胱氨酸天冬氨酸蛋白酶3（Caspase-3）、B 细胞淋巴瘤因子2（Bcl-2）、 Bcl-2 相关X 蛋白（Bax）、RhoA、ROCK1、ROCK2 蛋白表达水平检测。结果与对照组比较，模型组心肌组织 有大量蓝色胶原纤维沉积，左室舒张末期容积（left ventricular end-diastolic volume，LVEDV）、左室收缩末期容 积（left ventricular end-systolic volume，LVESV）、IL-17、Th17、Th17/Treg、ET-1、心肌梗死面积、细胞凋亡 率、TUNEL 阳性率、Bax、Caspase-3、RhoA 活性、RhoA、ROCK1、ROCK2 表达水平明显升高，左室射血分 数（left ventricular ejection fraction，LVEF）、左室缩短分数（left ventricular shortening fraction，LVFS）、TGF-β、 Treg、eNOS、Bcl-2 表达水平明显降低（P＜0.05）。与模型组比较，Matrine-L 组、苦参碱高剂量组心肌组织蓝 色胶原纤维逐渐减少，LVEDV、LVESV、IL-17、Th17、Th17/Treg、ET-1、心肌梗死面积、细胞凋亡率、 TUNEL 阳性率、Bax、Caspase-3、RhoA 活性、RhoA、ROCK1、ROCK2 表达水平依次明显降低， LVEF、 LVFS、TGF-β、Treg、eNOS、Bcl-2 表达水平依次明显升高（P＜0.05）。与苦参碱高剂量组比较，苦参碱高剂 量+LPA 组心肌组织蓝色胶原纤维增多，LVEDV、LVESV、IL-17、Th17、Th17/Treg、ET-1、心肌梗死面积、 细胞凋亡率、TUNEL 阳性率、Bax、Caspase-3、RhoA 活性、RhoA、ROCK1、ROCK2 表达水平明显升高， LVEF、LVFS、TGF-β、Treg、eNOS、Bcl-2 表达水平明显降低（P＜0.05）。结论苦参碱通过抑制RhoAROCK 信号通路调节Th17/Treg 细胞平衡，改善冠心病大鼠心肌损伤。

To investigate the impacts of matrine on the balance of helper T cell 17 （Th17）/regulatory T cell （Treg） and the Ras homolog gene family member A （RhoA）- Rho-associated coiled-coil forming protein kinase （ROCK） signaling pathway in coronary heart disease （CHD） rats. Methods A model of coronary heart disease was established. Rats were grouped into control group， model group （CHD group）， low-dose matrine （50 mg·kg-1，Matrine-L） group，high-dose matrine （200 mg·kg-1，Matrine-H） group，and Matrine-H+LPA （200 mg·kg-1 matrine+10 mg·kg-1 LPA）group. Echocardiography was applied to detect cardiac function. Enzyme linked immunosorbent assay （ELISA）method was used to detect interleukin-17 （IL-17） and transforming growth factor（TGF-β）. The quantity of Th17，Treg and Th17/Treg ratio were detected by flow cytometry. Immunohistochemistry was applied to detect the protein expressions of endothelial nitric oxide synthase （eNOS） and endothelin 1（ET-1）. Masson staining was carried out to observe the pathological changes of myocardial tissue. The myocardial infarction in each group of rats was observed by TCC staining. TUNEL staining was performed to detect cell apoptosis in myocardial tissue. Additionally，RhoA activity was detected by assay kit. Western Blot method was applied to detect the protein expressions levels of B-cell lymphoma factor 2 （Bcl-2），Bcl-2 associated X protein （Bax），cysteine aspartate proteinase-3 （Caspase-3）， RhoA， ROCK1 and ROCK2. Results Compared with the control group， a large amount of blue collagen fiber deposition was observed in the myocardial tissue of CHD group. The expression levels of left ventricular end-diastolic volume （LVEDV），left ventricular end-systolic volume （LVESV），IL-17， Th17， Th17 / Treg， ET-1， infarct size， cell apoptosis rate， TUNEL positive rate， Bax， Caspase-3， RhoA activity，RhoA，ROCK1，ROCK2 were obviously increased. The expression levels of left ventricular ejection fraction （LVEF），left ventricular shortening fraction （LVFS），TGF-β，Treg，eNOS，and Bcl-2 were obviously reduced （P＜0.05） . Compared with the CHD group，blue collagen fibers in myocardial tissue of Matrine-L and Matrine-H groups gradually decreased. The expression levels of LVEDV，LVESV，IL-17，Th17，Th17/Treg，ET-1，infarct size， cell apoptosis rate， TUNEL positive rate， Bax， Caspase-3， RhoA activity， RhoA， ROCK1 and ROCK2 were obviously reduced in sequence. The expression levels of LVEF， LVFS， TGF-β， Treg， eNOS， and Bcl-2 were also obviously increased in sequence （P＜0.05） . Compared with the Matrine-H group，blue collagen fibers in myocardial tissue of Matrine-H+LPA group increased. The expression levels of LVEDV，LVESV，IL-17，Th17， Th17 / Treg， ET-1， infarct size， cell apoptosis rate， TUNEL positive rate， Bax， Caspase-3， RhoA activity， RhoA，ROCK1，ROCK2 were obviously increased，while the expression levels of LVEF，LVFS，TGF-β，Treg， eNOS and Bcl-2 were obviously reduced （P＜0.05） . Conclusion Matrine regulates Th17/Treg cell balance and improves myocardial injury in rats with CHD by inhibiting the RhoA-ROCK signaling pathway

R285.5

内蒙古自治区教育厅资助项目（NJZZ21040）。